Dihydrotestosterone and dihydrotestosterone/ratio may be used for differential diagnosis of diseases of sexual differentiation (DSD). DHT levels may be inappropriately low compared to testosterone levels in 5a-reductase deficiency. It is recommended by guidelines to measure the ratio of testosterone to DHT.
DHT levels may also be monitored to follow the effectiveness of 5a-reductase inhibitor tesatment in female alopecia.
High-pressure liquid chromatography (HPLC) with tandem mass spectrometry