Sexually transmitted diseases (STDs) are markers of high risk sexual behavior. Herpes simplex virus (HSV) types 1 and 2 cause genital herpes infections and are the most common cause of genital ulcer disease worldwide.[1,2] Considering that herpes is a life long infection, not cured by antimicrobial treatment, HSV-2 antibodies are a much more reliable indicator of risky behavior than Treponema palladium antibodies.[3,4] As large number of genital infections are also caused by HSV-1. The present study was thus undertaken for finding IgM antibodies against HSV-1 and 2.While most herpetic infections are asymptomatic or mild, some can be transmitted to neonates and are associated with other STDs and cervical neoplasia. Genital herpes may contribute more to human immunodeficiency virus (HIV) infection because of its higher frequency than other STDs, the recurrence of genital herpes and large number of herpes infected persons who continue their sexual activities despite being infectious.Serology is the only practical way to diagnose HSV infection in individuals without any relevant clinical history or presentation with lesionsImmunoglobulin M (IgM) antibodies to HSV are increased to four times the normal value 2–4 weeks after the infection and the enzyme linked immuno sorbent assay (ELISA) is a specific, sensitive, and simple test which confirms the infection by HSV.